LMSF LacI - Pilot F005
GROQ-seq function measurements for LacI transcription factor pilot
About This Dataset
The project aims to generate sequence → function data for allosteric transcription factors, supporting applications in synthetic biology, biosensors, and therapeutics.
This dataset contains GROQ-seq measurements of LacI variant libraries (SSVL, epPCR, and SS) assayed against the wild-type operator at the Living Measurement Systems Foundry (LMSF) at NIST, with a companion dataset at DAMP (Boston University) for cross-site reproducibility.
Dataset Information
- Owning Organization
- Wyss Institute
- Gene
- LacI
- Released
- Mar 16, 2026
Experiment Details
- total Records
- 44,925
- host Organism
- Escherichia coli
- antibiotic
- Trimethoprim
- experiment Date
- 6/22/2025
- collection Site
- LMSF
Downloads
TF_LMSF_LacI_output_v1.zip
Final output file containing function values
4.2 MB
TF_LMSF_Supplemental_v1.zip
Supplemental data including QC analysis notebooks
533.2 MB
README.md
Dataset README with project background and methodology overview
14.3 KB
Uncertainty Estimation Methodology.md
Uncertainty estimation methodology used for function measurements
10.6 KB
Figures
Reproducibility plot showing correlation between LMSF (NIST) and DAMP collection sites for LacI (plotting the log_g_2000_IPTG values)