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T7RNAP - Pilot
GROQ-seq function measurements for T7RNAP SSVL and epPCR libraries
About This Dataset
The project aims to collect data on thousands of polymerase sequence variants, with applications in protein engineering for synthetic biology, mRNA therapeutics, and biosensors.
This dataset contains GROQ-seq measurements of T7 RNA polymerase site-saturation (SSVL) and error-prone PCR (epPCR) libraries — 34,000 variants assayed via mDHFR-mediated growth at the DAMP Laboratory (Boston University).
Dataset Information
- Owning Organization
- Crick BioDesign Lab
- Gene
- T7RNAP
- Released
- Mar 23, 2026
Experiment Details
- total Records
- 34,651
- host Organism
- Escherichia coli
- antibiotic
- Trimethoprim
- experiment Date
- 11/11/2025
- collection Site
- DAMP