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TEV Protease - Pilot SSVL and epPCR Libraries

GROQ-seq function measurements for TEV protease SSVL and epPCR libraries

About This Dataset

The project aims to collect data on thousands of protease sequence variants, with applications in understanding protease specificity and developing therapeutic interventions.

This dataset contains GROQ-seq measurements of TEV protease site-saturation (SSVL) and error-prone PCR (epPCR) libraries — over 18,000 variants assayed via a split-DHFR system across 24 conditions at the Living Measurement Systems Foundry (LMSF) at NIST.

Dataset Information

Owning Organization
The Align Foundation
Gene
TEV
Released
May 12, 2026

Experiment Details

total Records
18,486
host Organism
Escherichia coli
antibiotic
Trimethoprim
experiment Date
8/21/2025
collection Site
LMSF

Downloads

TEV_Pilot_SSVL_EP_output_v1.1.zip

File containing final function values for variants

19.7 MB

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TEV_Pilot_SSVL_EP_Supplemental_v1.1.zip

Supplemental data including QC analysis notebooks

2.1 GB

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README.md

Dataset README

4.9 KB

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